Pain panel extracted from human urine at cutoff - Kinetex C18 2.6µm 50x2.1mm LCMSMS

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid in H2O

B: 0.1% Formic acid in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

1.3

40

60

3

1.31

5

95

4

1.51

5

95

5

1.52

95

5

6

2.2

95

5

Flow Rate

1.20 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S029-TBJ

Condition 1

1 mL methanol

Condition 2

1 mL 100 mM ammonium acetate buffer (pH 5.95)

Load

Load Sample

Wash 1

2 x 1 mL 100 mM ammonium acetate buffer (pH 5.95)

Wash 2

2 x 1 mL methanol

Dry

5 mins under full vacuum

Elution 1

2 x 0.5 mL 60:24:16 ethyl acetate / IPA / NH4OH

Evaporation

Evaporate to dryness

Reconstitute

1 mL 1 mL Initial Mobile Phase