Glibenclamide Related Substance HPLC Analysis (HP-EUR) on Kinetex 2.6u C18 100 x 4.6mm ID

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 20:50:930 101.8g/L Triethylamine solution/ACN/H2O

B: 20:65:915 mobile phase A/H2O/ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

45

55

2

15

45

55

3

30

5

95

4

40

5

95

5

41

45

55

6

55

45

55

Flow Rate

0.80 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info