USP 621: Allowable changes in flow rate- 1.0 mL/Min

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4252-E0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

45

55

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282