Tricyclic Antidepressant on Kinetex 2.6µm C8 by LC/MS/MS

LC Conditions

Column

Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4497-AN

Phase Name

C8

Elution Type

Gradient

Mobile Phase

A: 0.1% Aq Formic Acid

B: 100% Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

3

40

60

3

4

40

60

4

4.1

10

90

5

5

10

90

6

5.1

85

15

Flow Rate

0.40 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info