LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (10 ng/mL) using Strata-X and Kinetex C8
LC Conditions
Column
Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Brand
Kinetex
Part No
00B-4497-AN
Phase Name
C8
Elution Type
Gradient
Mobile Phase
A: 10mM Ammonium acetate
B: 10mM Ammonium acetate in methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
50
50
2
2.5
0
100
3
2.51
50
50
4
5
50
50
Flow Rate
400.00 mL/min
Temperature
0°C
Detection
Mass Spectrometer (MS) @ (350°C)
Detection Info
Sample Preparation Details
Description
Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk
Part No
8B-S100-TBJ
Condition 1
2 mL Methanol at 2 mL/min
Condition 2
2 mL 10mM Ammonium acetate at 2 mL/min
Load
Load 3.00 mL Sample diluted in Water
Wash 1
1 mL 50:50 10mM ammonium acetate/10mM ammonium acetate in methanol at 2 mL/min
Dry
10min at full vacuum
Elution 1
2 mL Methanol at 1 mL/min
Evaporation
Evaporate to dryness
Reconstitute
100 µL 50:50 Methanol/water
Order Items Used in This Application
- 1. Digoxin
- 2. Oleandrin
- 3. Digitoxin
-OEChem-05081000432D<br><br>119126 0 1 0 0 0 0 0999 V2000<br> 18.3157 -1.5584 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 17.4497 1.9416 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.9226 -2.6107 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 19.5754 3.3149 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0624 -1.1074 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 21.3360 3.5906 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4586 -2.5973 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4663 -0.5973 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5945 -2.0940 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9945 -2.5840 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0022 -0.5840 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2625 -2.5773 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2740 0.4227 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5381 -0.5706 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.3157 -0.5584 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 17.4497 -1.0584 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 16.5837 -0.5584 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 18.3157 0.4416 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 15.6737 -1.0652 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 15.6656 -2.1068 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 17.4497 0.9416 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 16.5837 0.4416 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 19.2619 0.7464 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 19.2619 -0.8631 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 17.4658 -2.0999 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.5677 -2.6276 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 19.8456 -0.0584 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.7468 -0.5011 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.3157 1.4416 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.7302 -2.6565 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.6814 -0.0652 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.7990 -1.0291 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.7906 -2.1140 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 19.5726 1.6969 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.9862 2.5069 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 20.5242 2.0043 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 20.5259 3.0043 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0586 -2.1074 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 11.1906 -2.6040 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3265 -2.1007 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 11.1983 -0.6040 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 10.3304 -1.1007 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 11.2022 0.3960 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5983 -1.0940 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.7342 -0.5907 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8663 -1.0873 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.7265 -2.5907 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.8624 -2.0873 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.7227 -3.5906 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1304 -2.0806 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 5.1343 -1.0807 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2702 -0.5773 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 3.3984 -2.0740 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 3.4022 -1.0740 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 2.5304 -2.5706 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.1891 -1.4777 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 17.3198 -0.1334 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.6689 -2.9568 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.9128 1.2516 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.3716 1.0243 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.9731 0.3340 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 19.8744 0.8426 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 19.0109 -1.4300 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 19.7993 -1.1723 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 17.6838 -2.6803 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.0749 -1.9844 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.1704 -3.1036 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.9687 -3.1005 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 20.3064 -0.4731 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 20.3064 0.3564 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.1550 -0.0345 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.3569 -0.0191 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.9357 1.4416 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.3157 2.0616 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 17.6957 1.4416 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.3329 -3.1325 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.1312 -3.1294 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.3014 -0.0700 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.6862 0.5548 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.0614 -0.0605 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.5944 -0.4438 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.1873 -1.1304 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.2555 -1.8009 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.8527 -1.8684 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.9128 2.2516 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.5262 2.9226 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 18.5248 2.0929 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 21.0252 1.6390 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0562 -2.7274 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.7903 -3.0774 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.5873 -3.0805 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.7908 -1.7886 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.7364 -0.2961 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3328 -0.4807 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.8222 0.3936 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.2046 1.0160 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5822 0.3984 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4562 -3.2173 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6007 -0.4740 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.1346 -0.1173 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3376 -0.1142 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8687 -0.4673 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.2623 -2.9027 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8600 -2.7073 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1027 -3.5883 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7203 -4.2106 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3427 -3.5930 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0046 0.0360 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1280 -2.7006 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7444 -1.1907 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3486 -0.4989 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7344 -0.2653 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3960 -2.6940 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8641 -1.3819 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2225 -2.0325 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.9923 -2.8786 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8384 -3.1088 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7383 0.7347 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.8786 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15 1 1 1 0 0 0<br> 1 84 1 0 0 0 0<br> 21 2 1 1 0 0 0<br> 2 85 1 0 0 0 0<br> 33 3 1 1 0 0 0<br> 38 3 1 1 0 0 0<br> 4 35 1 0 0 0 0<br> 4 37 1 0 0 0 0<br> 5 38 1 0 0 0 0<br> 5 41 1 0 0 0 0<br> 6 37 2 0 0 0 0<br> 40 7 1 6 0 0 0<br> 7 98 1 0 0 0 0<br> 42 8 1 6 0 0 0<br> 44 8 1 6 0 0 0<br> 9 44 1 0 0 0 0<br> 9 47 1 0 0 0 0<br> 48 10 1 1 0 0 0<br> 50 10 1 6 0 0 0<br> 46 11 1 1 0 0 0<br> 11108 1 0 0 0 0<br> 12 50 1 0 0 0 0<br> 12 53 1 0 0 0 0<br> 52 13 1 1 0 0 0<br> 13118 1 0 0 0 0<br> 54 14 1 1 0 0 0<br> 14119 1 0 0 0 0<br> 15 16 1 0 0 0 0<br> 15 18 1 0 0 0 0<br> 15 24 1 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 25 1 0 0 0 0<br> 16 56 1 1 0 0 0<br> 17 19 1 0 0 0 0<br> 17 22 1 0 0 0 0<br> 17 57 1 6 0 0 0<br> 18 21 1 0 0 0 0<br> 18 23 1 0 0 0 0<br> 18 29 1 1 0 0 0<br> 19 20 1 0 0 0 0<br> 19 28 1 0 0 0 0<br> 19 31 1 1 0 0 0<br> 20 26 1 0 0 0 0<br> 20 30 1 0 0 0 0<br> 20 58 1 1 0 0 0<br> 21 22 1 0 0 0 0<br> 21 59 1 0 0 0 0<br> 22 60 1 0 0 0 0<br> 22 61 1 0 0 0 0<br> 23 27 1 0 0 0 0<br> 23 34 1 0 0 0 0<br> 23 62 1 0 0 0 0<br> 24 27 1 0 0 0 0<br> 24 63 1 0 0 0 0<br> 24 64 1 0 0 0 0<br> 25 26 1 0 0 0 0<br> 25 65 1 0 0 0 0<br> 25 66 1 0 0 0 0<br> 26 67 1 0 0 0 0<br> 26 68 1 0 0 0 0<br> 27 69 1 0 0 0 0<br> 27 70 1 0 0 0 0<br> 28 32 1 0 0 0 0<br> 28 71 1 0 0 0 0<br> 28 72 1 0 0 0 0<br> 29 73 1 0 0 0 0<br> 29 74 1 0 0 0 0<br> 29 75 1 0 0 0 0<br> 30 33 1 0 0 0 0<br> 30 76 1 0 0 0 0<br> 30 77 1 0 0 0 0<br> 31 78 1 0 0 0 0<br> 31 79 1 0 0 0 0<br> 31 80 1 0 0 0 0<br> 32 33 1 0 0 0 0<br> 32 81 1 0 0 0 0<br> 32 82 1 0 0 0 0<br> 33 83 1 0 0 0 0<br> 34 35 1 0 0 0 0<br> 34 36 2 0 0 0 0<br> 35 86 1 0 0 0 0<br> 35 87 1 0 0 0 0<br> 36 37 1 0 0 0 0<br> 36 88 1 0 0 0 0<br> 38 39 1 0 0 0 0<br> 38 89 1 0 0 0 0<br> 39 40 1 0 0 0 0<br> 39 90 1 0 0 0 0<br> 39 91 1 0 0 0 0<br> 40 42 1 0 0 0 0<br> 40 92 1 0 0 0 0<br> 41 42 1 0 0 0 0<br> 41 43 1 1 0 0 0<br> 41 93 1 0 0 0 0<br> 42 94 1 0 0 0 0<br> 43 95 1 0 0 0 0<br> 43 96 1 0 0 0 0<br> 43 97 1 0 0 0 0<br> 44 45 1 0 0 0 0<br> 44 99 1 0 0 0 0<br> 45 46 1 0 0 0 0<br> 45100 1 0 0 0 0<br> 45101 1 0 0 0 0<br> 46 48 1 0 0 0 0<br> 46102 1 0 0 0 0<br> 47 48 1 0 0 0 0<br> 47 49 1 6 0 0 0<br> 47103 1 0 0 0 0<br> 48104 1 0 0 0 0<br> 49105 1 0 0 0 0<br> 49106 1 0 0 0 0<br> 49107 1 0 0 0 0<br> 50 51 1 0 0 0 0<br> 50109 1 0 0 0 0<br> 51 52 1 0 0 0 0<br> 51110 1 0 0 0 0<br> 51111 1 0 0 0 0<br> 52 54 1 0 0 0 0<br> 52112 1 0 0 0 0<br> 53 54 1 0 0 0 0<br> 53 55 1 6 0 0 0<br> 53113 1 0 0 0 0<br> 54114 1 0 0 0 0<br> 55115 1 0 0 0 0<br> 55116 1 0 0 0 0<br> 55117 1 0 0 0 0<br>M END
Compound Name
Digoxin
CID
30322
Molecular Formula
C41H64O14
Molecular Weight
780
No. Hydrogen Bond Acceptors
14
No. Hydrogen Bond Donors
6
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