Water soluble vitamins on Synergi Hydro-RP

LC Conditions

Column

Synergi 2.5 µm Hydro-RP 100 Å, LC Column 100 x 3 mm, Ea

Brand

Synergi

Part No

00D-4387-Y0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in DI H2O

B: 100% ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

2

98

2

3

5

40

60

4

6

40

60

5

6.1

10

90

6

8

10

90

7

8.1

98

2

Flow Rate

0.50 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info