Ethyl Glucuronide (25ng/mL) on Synergi Hydro-RP (m/z 221.1>75.0)

LC Conditions

Column

Synergi 2.5 µm Hydro-RP 100 Å, LC Column 100 x 3 mm, Ea

Brand

Synergi

Part No

00D-4387-Y0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: 10mM Ammonium formate

B: 50:50 Methanol: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

4

20

80

3

4.1

100

0

4

7

100

0

Flow Rate

0.50 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info