Phospholipid Removal Phree vs. Traditional PPT(Zoomed Scale)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

40

60

2

0.5

5

95

3

15.5

5

95

4

15.51

40

60

5

19.5

40

60

Flow Rate

400.00 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (425°C)

Detection Info

Sample Notes

Extracted sample was prepped with Phenomenex Phree Phospholipid Removal Plate. Step 1: Add 300uL of 1% formic acid in acetonitrile directly to Phree plate. Step 2: Add 100uL of plasma directly to Phree plate. Vortex and extract. This was compared to standard protein precipitated plasma using the same volume of plasma and crash solvent

Sample Preparation Details

Description

Phree Phospholipid Removal, 30 mg / well, 96-Well Plates, 2/Pk

Part No

8E-S133-TGB

Load

Load Sample

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase