Malachite Green and Related Triphenylmethane Dyes in Aquaculture Samples by LC/MS/MS

LC Conditions

Column

Synergi 4 µm Max-RP 80 Å, LC Column 50 x 2 mm, Ea

Brand

Synergi

Part No

00B-4337-B0

Phase Name

Max-RP

Elution Type

Gradient

Mobile Phase

A: 10mM Ammonium Acetate 0.1% formic acid

B: Acetonitrile/Water (90:10) with 10mM Ammonium acetate and 0.1% Formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

2

8

10

90

3

14

10

90

4

15

70

30

Flow Rate

0.40 mL/min

Temperature

30°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Notes

This separation uses IMethod Kit Part Number KH0-8986 SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 2.0mm ID, 10/Pk Part No.: AJ0-6073 - Holder Part No.: KJ0-4282