Sulfonamides in Meat by LC/MS/MS

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 50 x 4.6 mm, Ea

Brand

Gemini

Part No

00B-4439-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

2

98

2

3

2.1

85

15

4

6.5

50

50

5

7.1

0

100

6

8

0

100

7

8.1

98

2

8

10

98

2

Flow Rate

800.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (300°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S100-UBJ

Condition 1

4 mL Methanol

Condition 2

4 mL 0.1% Formic Acid in Water

Load

Load 4.00 mL Sample diluted in 0.1% Formic Acid/Methanol (95:5)

Wash 1

2 mL 0.1% Formic Acid in Water

Dry

2min at 10inches Hg

Elution 1

1 mL Methanol

Evaporation

Evaporate to dryness

Reconstitute

250 µL 90:10 water:methanol, containing 0.1% formic acid