Immunosuppressants by LCMS using Luna 5um Phenyl-Hexyl 50x2.1mm
LC Conditions
Column
Luna 5 µm Phenyl-Hexyl 100 Å, LC Column 50 x 2 mm, Ea
Brand
Luna
Part No
00B-4257-B0
Phase Name
Phenyl-Hexyl
Elution Type
Isocratic
Mobile Phase
A: 97% methanol + 0.3 mM ammonium acetate + 0.003% acetic acid
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
100
0
Flow Rate
0.50 mL/min
Temperature
22°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Everolimus
- 2. Sirolimus
- 3. Tacrolimus
- 4. Ascomycin
- 5. Cyclosporin D
-OEChem-04281017022D<br><br>151154 0 1 0 0 0 0 0999 V2000<br> 8.2865 -2.6318 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.1658 -1.9477 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.0811 3.4502 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.5434 -0.0888 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.6916 0.5792 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.2055 1.9192 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2526 2.2000 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.0194 2.3509 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2335 -4.3006 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.8113 1.9112 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.6377 -0.1040 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3100 2.1183 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.9410 1.7939 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1511 -0.0888 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.8113 2.9112 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8075 0.3227 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 9.5734 -0.3201 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8678 -0.0194 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9811 1.3075 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3998 -1.3049 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.4602 -1.6470 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.6941 -1.0042 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.2151 1.9502 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.2151 2.9502 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.3491 1.4502 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3491 3.4502 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9453 3.4112 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 13.5512 0.9292 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 12.6774 0.4112 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.3468 -2.9738 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.5434 -1.0888 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 14.4329 0.4247 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0793 2.9112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9453 4.4112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.4289 -0.5911 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9922 -2.9325 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3831 3.1914 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.6774 3.4112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.8113 4.9112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.6774 4.4112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.6774 -1.5888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.6774 1.4112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5171 3.6914 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 11.8113 1.9112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.1880 1.7002 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1732 -3.9586 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.8113 -1.0888 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 5.2583 4.6574 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0171 2.8254 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9453 -1.5888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0171 2.8254 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3100 2.1183 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 10.9453 -2.5888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0793 -1.0888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0171 1.4112 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 3.6344 3.7493 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.6980 0.2380 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0171 0.4112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8831 -0.0888 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 9.2133 -1.5888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7492 0.4112 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6152 -0.0888 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 4.8831 -1.0888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3472 -1.0888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6152 -1.0888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1152 0.7772 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4812 -1.5888 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7343 1.1851 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3901 0.5347 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.1560 -0.5322 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.8834 0.2168 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7601 0.5912 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2478 -0.0194 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5637 1.0954 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2911 1.8444 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9824 -1.0929 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9351 -2.0455 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1115 -0.7921 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3841 -1.5411 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6782 2.2602 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9512 2.5253 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.0391 1.9872 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8121 1.1402 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6591 0.9133 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7875 3.8887 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.0391 3.9872 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.2092 3.8362 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.1481 1.4003 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2392 -2.3632 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7268 -2.9738 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.9641 -1.8274 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.0433 0.3163 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.6442 1.0076 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.6357 -1.1756 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.0402 -0.4874 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.7333 4.9938 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3347 4.3035 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3816 -2.8249 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.8845 -3.5431 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.6028 -3.0402 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.8894 2.8286 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.2879 3.5189 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.2099 5.3861 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4128 5.3861 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.2879 4.3035 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.8894 4.9938 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.2788 -2.0638 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.0759 -2.0638 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0090 4.0689 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.6660 1.3054 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.5829 2.1782 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.7100 2.0951 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2809 -4.5692 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7932 -3.9586 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.3192 -0.7332 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.2144 0.9749 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.8572 4.8178 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0978 5.2562 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6594 4.4969 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3271 2.2885 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1258 -4.9112 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0727 1.5455 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3253 -2.5888 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9453 -3.2088 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.5653 -2.5888 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.1869 -0.4782 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5326 1.0667 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2072 3.9866 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3972 4.3221 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0616 3.5120 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9100 0.8206 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.1154 0.4501 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.4859 -0.3446 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3462 -0.3988 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2133 -2.2088 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1477 0.8861 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3506 0.8861 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 2.6552 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2141 -0.2493 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2631 -1.0888 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8831 -1.7088 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5031 -1.0888 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3472 -0.4688 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0782 -1.3988 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6521 0.4672 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4252 1.3142 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5782 1.0872 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4812 -2.2088 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3569 0.6932 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2262 0.8077 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1118 1.6770 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 21 1 1 6 0 0 0<br> 1 30 1 0 0 0 0<br> 20 2 1 1 0 0 0<br> 2 36 1 0 0 0 0<br> 3 24 1 0 0 0 0<br> 3 33 1 0 0 0 0<br> 4 29 1 0 0 0 0<br> 4 31 1 0 0 0 0<br> 29 5 1 6 0 0 0<br> 5116 1 0 0 0 0<br> 6 33 2 0 0 0 0<br> 7 37 2 0 0 0 0<br> 8 42 2 0 0 0 0<br> 9 46 1 0 0 0 0<br> 9121 1 0 0 0 0<br> 10 44 2 0 0 0 0<br> 47 11 1 1 0 0 0<br> 11 57 1 0 0 0 0<br> 52 12 1 6 0 0 0<br> 12138 1 0 0 0 0<br> 55 13 1 1 0 0 0<br> 13 68 1 0 0 0 0<br> 14 58 2 0 0 0 0<br> 15 27 1 0 0 0 0<br> 15 38 1 0 0 0 0<br> 15 44 1 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 18 1 0 0 0 0<br> 16 19 1 1 0 0 0<br> 16 69 1 0 0 0 0<br> 17 20 1 0 0 0 0<br> 17 70 1 0 0 0 0<br> 17 71 1 0 0 0 0<br> 18 22 1 0 0 0 0<br> 18 72 1 0 0 0 0<br> 18 73 1 0 0 0 0<br> 19 23 1 0 0 0 0<br> 19 74 1 0 0 0 0<br> 19 75 1 0 0 0 0<br> 20 21 1 0 0 0 0<br> 20 76 1 0 0 0 0<br> 21 22 1 0 0 0 0<br> 21 77 1 0 0 0 0<br> 22 78 1 0 0 0 0<br> 22 79 1 0 0 0 0<br> 23 24 1 0 0 0 0<br> 23 25 1 1 0 0 0<br> 23 80 1 0 0 0 0<br> 24 26 1 0 0 0 0<br> 24 81 1 1 0 0 0<br> 25 82 1 0 0 0 0<br> 25 83 1 0 0 0 0<br> 25 84 1 0 0 0 0<br> 26 37 1 0 0 0 0<br> 26 85 1 0 0 0 0<br> 26 86 1 0 0 0 0<br> 27 33 1 0 0 0 0<br> 27 34 1 0 0 0 0<br> 27 87 1 1 0 0 0<br> 28 29 1 0 0 0 0<br> 28 32 1 0 0 0 0<br> 28 45 1 6 0 0 0<br> 28 88 1 0 0 0 0<br> 29 42 1 0 0 0 0<br> 30 46 1 0 0 0 0<br> 30 89 1 0 0 0 0<br> 30 90 1 0 0 0 0<br> 31 35 1 0 0 0 0<br> 31 41 1 0 0 0 0<br> 31 91 1 6 0 0 0<br> 32 35 1 0 0 0 0<br> 32 92 1 0 0 0 0<br> 32 93 1 0 0 0 0<br> 34 39 1 0 0 0 0<br> 34 96 1 0 0 0 0<br> 34 97 1 0 0 0 0<br> 35 94 1 0 0 0 0<br> 35 95 1 0 0 0 0<br> 36 98 1 0 0 0 0<br> 36 99 1 0 0 0 0<br> 36100 1 0 0 0 0<br> 37 43 1 0 0 0 0<br> 38 40 1 0 0 0 0<br> 38101 1 0 0 0 0<br> 38102 1 0 0 0 0<br> 39 40 1 0 0 0 0<br> 39103 1 0 0 0 0<br> 39104 1 0 0 0 0<br> 40105 1 0 0 0 0<br> 40106 1 0 0 0 0<br> 41 47 1 0 0 0 0<br> 41107 1 0 0 0 0<br> 41108 1 0 0 0 0<br> 42 44 1 0 0 0 0<br> 43 48 1 1 0 0 0<br> 43 49 1 0 0 0 0<br> 43109 1 0 0 0 0<br> 45110 1 0 0 0 0<br> 45111 1 0 0 0 0<br> 45112 1 0 0 0 0<br> 46113 1 0 0 0 0<br> 46114 1 0 0 0 0<br> 47 50 1 0 0 0 0<br> 47115 1 0 0 0 0<br> 48117 1 0 0 0 0<br> 48118 1 0 0 0 0<br> 48119 1 0 0 0 0<br> 49 51 2 0 0 0 0<br> 49120 1 0 0 0 0<br> 50 53 1 0 0 0 0<br> 50 54 2 0 0 0 0<br> 51 52 1 0 0 0 0<br> 51 56 1 0 0 0 0<br> 52 55 1 0 0 0 0<br> 52122 1 0 0 0 0<br> 53123 1 0 0 0 0<br> 53124 1 0 0 0 0<br> 53125 1 0 0 0 0<br> 54 60 1 0 0 0 0<br> 54126 1 0 0 0 0<br> 55 58 1 0 0 0 0<br> 55127 1 0 0 0 0<br> 56128 1 0 0 0 0<br> 56129 1 0 0 0 0<br> 56130 1 0 0 0 0<br> 57131 1 0 0 0 0<br> 57132 1 0 0 0 0<br> 57133 1 0 0 0 0<br> 58 59 1 0 0 0 0<br> 59 61 1 0 0 0 0<br> 59 63 1 1 0 0 0<br> 59134 1 0 0 0 0<br> 60 64 2 0 0 0 0<br> 60135 1 0 0 0 0<br> 61 62 1 0 0 0 0<br> 61136 1 0 0 0 0<br> 61137 1 0 0 0 0<br> 62 65 1 0 0 0 0<br> 62 66 1 6 0 0 0<br> 62139 1 0 0 0 0<br> 63140 1 0 0 0 0<br> 63141 1 0 0 0 0<br> 63142 1 0 0 0 0<br> 64 67 1 0 0 0 0<br> 64143 1 0 0 0 0<br> 65 67 2 0 0 0 0<br> 65144 1 0 0 0 0<br> 66145 1 0 0 0 0<br> 66146 1 0 0 0 0<br> 66147 1 0 0 0 0<br> 67148 1 0 0 0 0<br> 68149 1 0 0 0 0<br> 68150 1 0 0 0 0<br> 68151 1 0 0 0 0<br>M END
Compound Name
Everolimus
CID
6442177
Molecular Formula
C53H83NO14
Molecular Weight
958
No. Hydrogen Bond Acceptors
14
No. Hydrogen Bond Donors
3
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Reversed Phase
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Reversed Phase
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