Pain Panel Opiates by LC/MS using Kinetex 2.6u C18 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 10mM ammonium formate

B: Methanol with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

3

5

95

3

3.1

95

5

4

5

95

5

Flow Rate

500.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info