Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm Wavelength Study

LC Conditions

Column

Kinetex 5 µm XB-C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4605-E0

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid

B: Acetonitrile 100%

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

3.5

100

0

3

3.6

0

100

4

5

0

100

5

5.1

100

0

6

7

100

0

Flow Rate

800.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Guard column and cartridges were used for human plasma vitamin C analysis: SecurityGuard Ultra Cartridges: Cat log No. AJO-8768. SecurityGuard Ultra Cartridge holder: Cat log No. AJO-9000.

Sample Preparation Details

Description

Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk

Part No

CE0-7565

Load

Load Sample

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase