PLP and PA in Human sera/plasma on Gemini-NX 3u, C18, 100x4.6 by HPLC w Fluorescence Detection-3

LC Conditions

Column

Gemini 3 µm NX-C18 110 Å, LC Column 100 x 4.6 mm, Ea

Brand

Gemini

Part No

00D-4453-E0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 20mmol/L Na2HPO4 + 1mL acetic acid in 1L DI Water

B: ACN : MeOH (70:30)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5

40

60

3

5.1

5

95

4

6

95

5

5

7

95

5

Flow Rate

1.00 mL/min

Temperature

35°C

Detection

Fluorescence (FLUOR) @ (22°C)

Detection Info