Opiates using Kinetex 2.6u Biphenyl 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4622-AN

Phase Name

Biphenyl

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% Formic acid

B: Methanol with 0.1% Formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

4

5

95

3

4.5

5

95

4

4.51

95

5

5

6

95

5

Flow Rate

0.50 mL/min

Temperature

40°C

Detection

Electrospray Mass Spec (ESMS) @ (22°C)

Detection Info