Cortisol/Cortisone Chromatogram_QCH (1600 ng/mL)
LC Conditions
Column
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
Brand
Kinetex
Part No
00B-4622-Y0
Phase Name
Biphenyl
Elution Type
Gradient
Mobile Phase
A: 10 mM ammonium acetate in water
B: 10 mM ammonium acetate in MeOH
Gradient Profile
Step No.
Time(min)
%A
%B
1
0.01
60
40
2
0.5
60
40
3
2
10
90
4
3
10
90
5
3.01
60
40
6
5
60
40
Flow Rate
0.40 mL/min
Temperature
40°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Cortisone
- 2. Cortisol
- 3. Prednisone
- 4. Prednisolone
-OEChem-04281015332D<br><br> 54 57 0 1 0 0 0 0 0999 V2000<br> 8.0319 1.8625 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7950 1.1062 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.0465 2.4920 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.8722 1.6754 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -2.4462 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 -0.3938 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.3931 0.6062 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.5271 -0.8938 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 5.6610 -0.3938 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.3393 0.9109 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 4.7510 -0.9007 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.3393 -0.6986 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5271 1.1062 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9229 0.1062 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5431 -1.9353 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6610 0.6062 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 1.6062 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7430 -1.9423 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6451 -2.4631 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8242 -0.3366 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7587 0.0993 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1493 1.4973 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8763 -0.8645 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8076 -2.4920 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8680 -1.9495 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0622 1.0890 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4830 -1.2390 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2664 -1.3131 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3972 0.0312 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0883 -1.2655 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8767 -1.0078 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9256 1.5811 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1285 1.5811 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3838 -0.3086 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3838 0.5209 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7612 -2.5157 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1523 -1.8198 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0131 1.6062 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 2.2262 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7731 1.6062 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2478 -2.9390 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0460 -2.9360 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2324 0.1301 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4343 0.1455 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3787 0.0945 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7635 0.7193 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1388 0.1041 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.4476 2.3225 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.6718 -0.2792 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2647 -0.9659 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8100 -3.1120 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.7146 0.5756 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5074 0.6575 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4382 1.4222 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10 1 1 6 0 0 0<br> 1 48 1 0 0 0 0<br> 2 16 2 0 0 0 0<br> 3 22 2 0 0 0 0<br> 4 26 1 0 0 0 0<br> 4 54 1 0 0 0 0<br> 5 25 2 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 8 1 0 0 0 0<br> 6 12 1 0 0 0 0<br> 6 27 1 6 0 0 0<br> 7 10 1 0 0 0 0<br> 7 13 1 0 0 0 0<br> 7 17 1 1 0 0 0<br> 8 9 1 0 0 0 0<br> 8 15 1 0 0 0 0<br> 8 28 1 1 0 0 0<br> 9 11 1 0 0 0 0<br> 9 16 1 0 0 0 0<br> 9 29 1 6 0 0 0<br> 10 14 1 0 0 0 0<br> 10 22 1 0 0 0 0<br> 11 18 1 0 0 0 0<br> 11 20 1 0 0 0 0<br> 11 21 1 1 0 0 0<br> 12 14 1 0 0 0 0<br> 12 30 1 0 0 0 0<br> 12 31 1 0 0 0 0<br> 13 16 1 0 0 0 0<br> 13 32 1 0 0 0 0<br> 13 33 1 0 0 0 0<br> 14 34 1 0 0 0 0<br> 14 35 1 0 0 0 0<br> 15 19 1 0 0 0 0<br> 15 36 1 0 0 0 0<br> 15 37 1 0 0 0 0<br> 17 38 1 0 0 0 0<br> 17 39 1 0 0 0 0<br> 17 40 1 0 0 0 0<br> 18 19 1 0 0 0 0<br> 18 24 2 0 0 0 0<br> 19 41 1 0 0 0 0<br> 19 42 1 0 0 0 0<br> 20 23 1 0 0 0 0<br> 20 43 1 0 0 0 0<br> 20 44 1 0 0 0 0<br> 21 45 1 0 0 0 0<br> 21 46 1 0 0 0 0<br> 21 47 1 0 0 0 0<br> 22 26 1 0 0 0 0<br> 23 25 1 0 0 0 0<br> 23 49 1 0 0 0 0<br> 23 50 1 0 0 0 0<br> 24 25 1 0 0 0 0<br> 24 51 1 0 0 0 0<br> 26 52 1 0 0 0 0<br> 26 53 1 0 0 0 0<br>M END
Compound Name
Cortisone
CID
222786
Molecular Formula
C21H28O5
Molecular Weight
360
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2
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