Vitamin B1 chromatogram of TDP, Thiamin and TMP

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 50 x 3 mm, Ea

Brand

Luna

Part No

00B-4252-Y0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 25 mM Na2HPO4 in water pH7.0

B: MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.01

95

5

2

1.8

85

15

3

3.5

50

50

4

4.5

50

50

5

4.51

95

5

6

6.5

95

5

Flow Rate

600.00 mL/min

Temperature

28°C

Detection

Fluorescence (FLUOR) @ (22°C)

Detection Info