Oxytocin Extracted Ion Chromatograms, QCL (0.500 ng/mL)

LC Conditions

Column

Luna 3 µm PFP(2) 100 Å, LC Column 50 x 2 mm, Ea

Brand

Luna

Part No

00B-4447-B0

Phase Name

PFP(2)

Elution Type

Gradient

Mobile Phase

A: 0.1%FA in water

B: 0.1%FA in 50:50 MeOH:Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

0.2

90

10

3

0.5

70

30

4

3.5

70

30

5

3.51

30

70

6

4

30

70

7

4.01

90

10

8

5

90

10

Flow Rate

0.45 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (40°C)

Detection Info

Sample Preparation Details

Description

Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / well, 96-Well Plates, 2/Pk

Part No

8E-S035-TGB

Condition 1

1 mL Methanol at 1 mL/min

Condition 2

1 mL Water at 1 mL/min

Sample Pretreatment

Strata - X - CW is selected, which works for both compounds

Load

Load 300.00 µL Sample diluted in 4% H3PO4 in water

Wash 1

1 mL 4% H3PO4 in water at 1 mL/min

Wash 2

1 mL 30% MeOH in water at 1 mL/min

Dry

3-5 min

Elution 1

0.75 mL 1% TFA in 50:50 MeOH: Acetonitrile at 0.75 mL/min

Elution 2

0.75 mL 1% TFA in 50:50 MeOH: Acetonitrile at 0.75 mL/min

Evaporation

Evaporate to dryness

Reconstitute

100 µL 1% TFA in 80:20 MPA:MPB