EPA 539 using Kinetex 5u EVO C18 100x3.0mm

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea

Brand

Kinetex

Part No

00D-4633-Y0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 0.2% ammonium hydroxide

B: Methanol with 0.2% ammonium hydroxide

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

65

35

2

0.6

35

65

3

7.5

35

65

4

8.5

15

85

5

13

15

85

6

13.01

35

65

7

15

35

65

Flow Rate

0.30 mL/min

Temperature

30°C

Detection

Electrospray Mass Spec (ESMS) @ (40°C)

Detection Info