Analysis of Parabens from Baby Wash on Kinetex 5u EVO C18 100x3.0

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea

Brand

Kinetex

Part No

00D-4633-Y0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 10% acetonitrile in water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

4

70

30

3

5

60

40

4

6

60

40

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Analytes 1. Methyl paraben 2. Ethyl paraben 3. Propyl paraben 4. Butyl paraben Novum SLE 12cc Tube 8B-S138-KDG Novum SLE 12cc Tube Extraction Procedure Sample pre-treatment 0.5 ± 0.05 g spiked Aveeno Baby (Wash & Shampoo) sample Add 1.25 mL acetone Add 0.6 ml 1% Nacl solution Vortex for 30 secs Wait 30 secs till the emulsion dissipates Centrifuge for 10 mins at 3000 rpm Collect the supernatant and proceed to load Sample loading "Load the sample from pre-treatment step above onto the Novum SLE cartridge and apply a short and gentle pulse of vacuum (~ 5" of Hg for 5-10 sec or until the sample has completely entered the media). "Wait for 5 minutes. Elution "Dispense 10 mL of ethylacetate onto the SLE media and allow the solvent to elute by gravity for 10 mins and collect the eluant. "Apply vacuum at 5"-10"of Hg to complete the extraction. NOTE: Prolonged application of vacuum will result in elution of body wash out of the SLE media and into the final extracted solvent. Dry down "Evaporate the final extract to complete dryness under slow stream of N2 at 45°C Reconstitute "Reconstitute the dry residue in 0.5 mL of methanol "Centrifuge for 5 mins at 6000 rpm "Collect supernatant to shoot on HPLC