| 1Amphetamine |
|---|
| 2Methamphetamine |
| 3MDMA |
| 4MDA |
| 5MDEA |
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Applications
Extraction of Amphetamines from Urine using Strata-X-C Microelution & Kinetex 2.6u Biphenyl, 50x2.1
22986
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Serotonin Agents
Extraction of Amphetamines from Urine using Strata-X-C Microelution & Kinetex 2.6u Biphenyl, 50x2.1
Analytes
Details
LC Conditions (App ID: 22986)
Column: |
Brand Name: Kinetex |
Part No: |
Phase Name: Biphenyl |
Sample Note: Extraction of Amphetamines from Human Urine Using Strata-X-C u-Elution Plate
Sorbent: Strata-X-C µ Elution 96-well Plate
Condition: 200 uL methanol
Equilibrate: 200 uL water
Load: 400 uL diluted human urine (200 uL sample diluted 1:1 with water)
Wash 1: 200 uL 2% formic acid/water
Wash 2: 200 uL methanol
Elute: 2x25uL 5% ammonium hydroxide/acetonitrile: methanol (60:40)
Direct injection: injection of 2 uL sample directly
HPLC:
Kinetex Biphenyl, 2.6u, 50x2.1; A=0.1% FA/water, B=0.1% FA/methanol;
Flow rate=0.5 mL/min; Inj. vol=2uL.
Step Total Time(min) Flow Rate (µl/min) A (%) B (%)
0 0.00 500 90.0 10.0
1 3.00 500 5.0 95.0
2 4.0 500 5.0 95.0
3 4.01 500 90.0 10.0
4 6.0 500 90.0 10.0 |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.