Extraction of Amphetamines from Urine using Strata-X-C Microelution & Kinetex 2.6u Biphenyl, 50x2.1
LC Conditions
Column
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
Brand
Kinetex
Part No
00B-4622-AN
Phase Name
Biphenyl
Elution Type
Gradient
Mobile Phase
A: 0.1% formic acid in water
B: 0.1% formic acid in methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
90
10
2
3
5
95
3
4
5
95
4
4.01
90
10
5
6
90
10
Flow Rate
0.50 mL/min
Temperature
25°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Sample Notes
Extraction of Amphetamines from Human Urine Using Strata-X-C u-Elution Plate Sorbent: Strata-X-C µ Elution 96-well Plate Condition: 200 uL methanol Equilibrate: 200 uL water Load: 400 uL diluted human urine (200 uL sample diluted 1:1 with water) Wash 1: 200 uL 2% formic acid/water Wash 2: 200 uL methanol Elute: 2x25uL 5% ammonium hydroxide/acetonitrile: methanol (60:40) Direct injection: injection of 2 uL sample directly HPLC: Kinetex Biphenyl, 2.6u, 50x2.1; A=0.1% FA/water, B=0.1% FA/methanol; Flow rate=0.5 mL/min; Inj. vol=2uL. Step Total Time(min) Flow Rate (µl/min) A (%) B (%) 0 0.00 500 90.0 10.0 1 3.00 500 5.0 95.0 2 4.0 500 5.0 95.0 3 4.01 500 90.0 10.0 4 6.0 500 90.0 10.0
Sample Preparation Details
Description
Novum SLE, MINI 96-Well Plate, 1/Pk
Part No
8E-S138-FGA
Pretreatment Note
Sample pre-treatment Dilute 150 uL of human plasma (spiked with 25 ng/mL and 125 ng/mL of cortisone and prednisolone respectively) with 150 uL of 50 mM sodium phosphate dibasic heptahydrate, pH unadjusted. Mix briefly (3-5 sec). Sample loading: Load the sample from pre-treatment step above onto the Novum plate and apply a short and gentle pulse of vacuum (~ 10" of Hg for 20 secs) until the sample has completely entered the media. Wait for 5 minutes. Elution: Dispense 1000 uL of ethyl acetate onto the SLE media and allow the solvent to elute by gravity (~ 5 min elution time) and collect the eluant. Apply vacuum at 5" of Hg for 45 secs to complete the extraction. NOTE: Prolonged application of vacuum will result in elution of plasma out of the SLE media and into the final extracted solvent. Dry down: Evaporate the final extract to complete dryness under slow stream of N2 at 40°C Reconstitute: Reconstitute the dry residue in 150 uL of initial mobile phase fortified with cortisol-D4.
Load
Load 150.00 µL Sample diluted in 150
Elution 1
1000 mL Ethyl acetate
Evaporation
Evaporate to dryness
Reconstitute
150 µL 10mM ammonium acetate in water:methanol(50:50)
Order Items Used in This Application
- 1. Amphetamine
- 2. Methamphetamine
- 3. MDMA
- 4. MDA
- 5. MDEA
-OEChem-06211003152D<br><br> 23 23 0 1 0 0 0 0 0999 V2000<br> 3.7320 2.0950 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.0950 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 2.8660 0.5950 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -0.4050 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 0.5950 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -0.9050 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.9050 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -1.9050 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.9050 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -2.4050 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.4682 1.5200 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.6540 1.1776 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2554 0.4873 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2881 0.0581 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 0.2850 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.9081 1.1319 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -0.5950 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 -0.5950 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 2.4050 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1951 2.4050 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -2.2150 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 -2.2150 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -3.0250 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 2 1 0 0 0 0<br> 1 19 1 0 0 0 0<br> 1 20 1 0 0 0 0<br> 2 3 1 0 0 0 0<br> 2 5 1 0 0 0 0<br> 2 11 1 0 0 0 0<br> 3 4 1 0 0 0 0<br> 3 12 1 0 0 0 0<br> 3 13 1 0 0 0 0<br> 4 6 2 0 0 0 0<br> 4 7 1 0 0 0 0<br> 5 14 1 0 0 0 0<br> 5 15 1 0 0 0 0<br> 5 16 1 0 0 0 0<br> 6 8 1 0 0 0 0<br> 6 17 1 0 0 0 0<br> 7 9 2 0 0 0 0<br> 7 18 1 0 0 0 0<br> 8 10 2 0 0 0 0<br> 8 21 1 0 0 0 0<br> 9 10 1 0 0 0 0<br> 9 22 1 0 0 0 0<br> 10 23 1 0 0 0 0<br>M END
Compound Name
Amphetamine
CID
3007
Molecular Formula
C9H13N
Molecular Weight
135
No. Hydrogen Bond Acceptors
1
No. Hydrogen Bond Donors
1
Similar Applications
Reversed Phase
DMPK: MDMA and Metabolites on Kinetex 2.6 µm Biphenyl by LC-MS/MS
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
26780
Reversed Phase
Analysis of Fat soluble Vitamins (A, E and K) Using a Kinetex 2.6u Biphenyl 50x3.0mm Column
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
26505
Reversed Phase
Extraction of Pain Panel (Drugs of Abuse) in Human Serum from Phree PLR Using a Kinetex 2.6u Biphenyl 50x3.0mm
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
26504
Reversed Phase
Fast Screening Method for 86 Forensic Analytes in Human Urine by LC-MS/MS
Luna 5 µm C18(2) 100 Å, LC Column 50 x 2 mm, Ea
25864
Reversed Phase
Pain Panel from Urine using Strata DE SLE and a Kinetex Biphenyl Core-Shell LC Column
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
24555
Reversed Phase
Workplace drug monitoring using Luna Omega 5um Polar C18 50x2.1mm
Luna Omega 5 µm Polar C18 100Å, LC Column 50 x 2.1 mm, EA
24156
Reversed Phase
Workplace drug monitoring using Luna Omega 3um Polar C18 50x2.1mm
Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 2.1 mm, Ea
24155
Reversed Phase
Workplace drug monitoring using Luna Omega 1.6um Polar C18 50x2.1mm
Luna Omega 1.6µm Polar C18 100Å, LC Column 50 x 2.1 mm, EA
24154
Chiral
Chiral Amphetamines with Ephedrines & Pseudoephedrines on Lux 3µm AMP 150x3mm
Lux 3 µm AMP, LC Column 150 x 3.0 mm, EA
23640
Chiral
Chiral Amphetamines Lux 3µm AMP 150x3mm
Lux 3 µm AMP, LC Column 150 x 3.0 mm, EA
23631
Reversed Phase
SAMHSA workplace drug testing panel using Luna Omega Polar C18 50x2.1mm
Luna Omega 1.6µm Polar C18 100Å, LC Column 50 x 2.1 mm, EA
23588
Reversed Phase
7.5 min Pain Panel on Kinetex 2.6µm Biphenyl, 30x2.1mm ESI+
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 30 x 2.1 mm, Ea
23560
Reversed Phase
Drugs of Abuse by LCMS using Kinetex 1.7u Biphenyl 50x2.1mm
Kinetex 1.7 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
23445
Reversed Phase
Drugs of Abuse by LCMS using Kinetex 1.7u EVO C18 50x2.1mm
Kinetex 1.7 µm EVO C18 100 Å, LC Column 50 x 2.1 mm, Ea
23444
Reversed Phase
Drugs of Abuse by LCMS using Luna Omega 1.6u C18 50x2.1mm
Luna Omega 1.6µm C18 100Å, LC Column 50 x 2.1 mm, EA
23443
Reversed Phase
Fast Pain Panel ESI+
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 30 x 2.1 mm, Ea
23384
Improved Performance
ZB-5MSplus Drugs of Abuse
Zebron ZB-5MSplus, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, EA
23258
Reversed Phase
Fast analysis of stimulants by LCMSMS using Kinetex 5u Biphenyl 50x2.1mm
Kinetex 5 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
22159
Reversed Phase
Stimulants by LCMS using Kinetex 5u Biphenyl 50x3.0mm
Kinetex 5 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
22151
Reversed Phase
Stimulants by LCMS using Kinetex 2.6u Biphenyl 50x3.0mm
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
22147
Reversed Phase
Full Pain Panel using Kinetex 2.6u Biphenyl 50x2.1mm
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
22122
Reversed Phase
Fast Pain Panel using Kinetex 2.6 u Biphenyl 50x3.0mm
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
22121
Reversed Phase
Full Pain Panel using Kinetex 5um Biphenyl 50x3.0mm
Kinetex 5 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
22120
Reversed Phase
Misc. Drugs of Abuse using Kinetex 2.6u Biphenyl 50x3.0mm
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
22110
Reversed Phase
Full Pain Panel using Kinetex 2.6u Biphenyl 50x3.0mm
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
22104
Reversed Phase
Pain Panel using Kinetex Phenyl Hexyl 2.6u 50x4.6mm
Kinetex 2.6 µm Phenyl-Hexyl 100 Å, LC Column 50 x 4.6 mm, Ea
20852
Reversed Phase
Pain Panel using Strata-X-Drug B and Kinetex Phenyl-Hexyl 2.6u 50x4.6mm
Kinetex 2.6 µm Phenyl-Hexyl 100 Å, LC Column 50 x 4.6 mm, Ea
20847
Reversed Phase
Bath salts analysis using Kinetex 2.6u C18 50x3.0mm
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 3 mm, Ea
20791
Reversed Phase
Bath Salts analysis using Kinetex 2.6u C18 50x3.0mm
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 3 mm, Ea
20739
Reversed Phase
Pain Panel/Drug Screening by LCMS using Kinetex 2.6u C18 50x3.0mm
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 3 mm, Ea
20689
Reversed Phase
Pain Panel Mix-41 in Human Urine on Luna 3u, PFP(2), 50x3 by LC-MS/MS
Luna 3 µm PFP(2) 100 Å, LC Column 50 x 3 mm, Ea
20594
Reversed Phase
Pain Panel Mix-41 in Human Urine on Kinetex 2.6u, PFP, 50x3 by LC-MS/MS
Kinetex 2.6 µm PFP 100 Å, LC Column 50 x 3 mm, Ea
20593
Reversed Phase
Pain Panel Mix-41 in Human Urine on Kinetex 2.6u, Phenyl-Hexyl, 50x2.1 by LC-MS/MS
Kinetex 2.6 µm Phenyl-Hexyl 100 Å, LC Column 50 x 2.1 mm, Ea
20592
Reversed Phase
Pain Panel Mix-41 in Human Urine on Kinetex 2.6u, XB-C18, 50x3 by LC-MS/MS
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 3 mm, Ea
20591
Reversed Phase
Pain Panel Mix-41 in Human Urine on Kinetex 2.6u, C18, 50x3 by LC-MS/MS
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 3 mm, Ea
20590
Reversed Phase
Amphetamines by LC/MS/MS using Strata-X-Drug B and a Kinetex C18 2.6µm, 50x2.1mm
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
19598
Reversed Phase
Kinetex SAMHSA LC/MS Run
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
19593
Reversed Phase
Amphetamines by LC/MS/MS using a Kinetex XB-C18 2.6µm, 50x2.1mm
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
19492
Reversed Phase
Amphetamines by LC/MS/MS using a Kinetex C8 2.6µm, 50x2.1mm
Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
19491
Reversed Phase
Amphetamines by LC/MS/MS using a Kinetex 2.6µm C18 50x2.1mm
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
19490
Reversed Phase
Expanded Amphetamines on Kinetex C18 2.6µm 50x2.1mm by LCMS
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
19166
Reversed Phase
NIDA-5 Extracted from Human Urine at Cutoff - Kinetex 2.6µm C18 50 x 2.1mm ID by LCMSMS
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
18851
Reversed Phase
Extraction of Expanded Amphetamines Using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
18847
Reversed Phase
Extraction of Amphetamines-NIDA5 Using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm by LCMS
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
18845
Application Specific
Alternative amphetamines by MSD on a ZB-Drug1 (acetyl derivative)
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
18512
Application Specific
Alternative amphetamines from urine Strata-XC on a ZB-Drug1 (acetyl derivative)
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
18511
Application Specific
Alternative amphetamines by MSD on a ZB-Drug1
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
18490
Application Specific
Expanded amphetamines from urine by Strata-XC on a ZB-Drug1
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
18482
Application Specific
Expanded amphetamines and interferences including TFMPP by MSD on a ZB-Drug1
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
18039
Application Specific
Expanded amphetamines from urine by Screen C on a ZB-Drug1
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
18034
Expanded Amphetamines and Interferences by MSD on a ZB-5MSi
17933
Application Specific
Expanded Amphetamines and Interferences by MSD on a ZB-Drug-1
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
17932
Reversed Phase
Amphetamines in Urine by LC/MS/MS on Gemini 5µm C18 150 x 3.0
Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea
17900
Standard
Expanded amphetamines by MSD on a ZB-5MS
Zebron ZB-5MS, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
17892
Application Specific
Expanded Amphetamines by MSD on a ZB-Drug-1
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
17809
Application Specific
Amphetamines by MSD on a ZB-Drug1
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
17807
Amphetamines by MSD on a ZB-5MSi
17793
Expanded amphetamines by MSD on a ZB-5MSi
17786
Expanded amphetamines by MSD on a ZB-1MS
17618
Amphetamines by MSD on a ZB-1MS
17617
Amphetamines by MSD on a ZB-XLB
17613
Expanded amphetamines by MSD on a ZB-XLB
17522
Reversed Phase
Extraction of Amphetamines on Strata-X-C
Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea
17192
Standard
Amphetamines by MSD on a ZB-5MS
Zebron ZB-5MS, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
16737
Amphetamines (PFPA Derivatization) on ZB1ms 20x0.18x0.18
15574
Standard
Amphetamines on ZB-5 30x0.25x0.25
Zebron ZB-5, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, Ea
14818
Standard
Amphetamines on ZB-5
Zebron ZB-5, GC Cap. Column 15 m x 0.25 mm x 0.25 µm, Ea
14784
Ion Exchange
Luna SCX Batch Reproducibility - Amphetamines (Batch 3)
Luna 5 µm SCX 100 Å, LC Column 150 x 4.6 mm, Ea
14563
Ion Exchange
Luna SCX Batch Reproducibility - Amphetamines (Batch 2)
Luna 5 µm SCX 100 Å, LC Column 150 x 4.6 mm, Ea
14561
Ion Exchange
Luna SCX Batch Reproducibility - Amphetamines (Batch 1)
Luna 5 µm SCX 100 Å, LC Column 150 x 4.6 mm, Ea
14559
Standard
Drugs of Abuse
Zebron ZB-1, GC Cap. Column 15 m x 0.25 mm x 0.25 µm, Ea
6567
Reversed Phase
Drugs of Abuse
Luna 5 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
1232
Standard
Drugs of Abuse
Zebron ZB-1, GC Cap. Column 15 m x 0.53 mm x 0.50 µm, Ea
1080