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Applications
Extraction of Amphetamines from Urine using Strata-X-C Microelution & Kinetex 2.6u Biphenyl, 50x2.1
22986
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Serotonin Agents

Extraction of Amphetamines from Urine using Strata-X-C Microelution & Kinetex 2.6u Biphenyl, 50x2.1

Analytes
1Amphetamine
2Methamphetamine
3MDMA
4MDA
5MDEA
Details
LC Conditions (App ID: 22986)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
Biphenyl
Sample Note:
Extraction of Amphetamines from Human Urine Using Strata-X-C u-Elution Plate Sorbent: Strata-X-C µ Elution 96-well Plate Condition: 200 uL methanol Equilibrate: 200 uL water Load: 400 uL diluted human urine (200 uL sample diluted 1:1 with water) Wash 1: 200 uL 2% formic acid/water Wash 2: 200 uL methanol Elute: 2x25uL 5% ammonium hydroxide/acetonitrile: methanol (60:40) Direct injection: injection of 2 uL sample directly HPLC: Kinetex Biphenyl, 2.6u, 50x2.1; A=0.1% FA/water, B=0.1% FA/methanol; Flow rate=0.5 mL/min; Inj. vol=2uL. Step Total Time(min) Flow Rate (µl/min) A (%) B (%) 0 0.00 500 90.0 10.0 1 3.00 500 5.0 95.0 2 4.0 500 5.0 95.0 3 4.01 500 90.0 10.0 4 6.0 500 90.0 10.0
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