Nicotine & Metabolites on Kinetex 2.6µm Evo C18 100x3mm

LC Conditions

Column

Kinetex 2.6 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, EA

Brand

Kinetex

Part No

00D-4725-Y0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 20mM Ammonium Bicarbonate, pH 8.2

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

3

10

90

3

5

10

90

4

5.01

90

10

5

6

90

10

Flow Rate

750.00 mL/min

Temperature

25°C

Detection

Mass Spectrometer (MS) @ (0°C)

Detection Info