Carcinogenic amines on Kinetex 2.6 µm EVO C18
LC Conditions
Column
Kinetex 2.6 µm EVO C18 100 Å, LC Column 150 x 2.1 mm, EA
Brand
Kinetex
Part No
00F-4725-AN
Phase Name
EVO C18
Elution Type
Gradient
Mobile Phase
A: 10 mM ammonium acetate/ ACN (95:5)
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
2
10
5
95
Flow Rate
0.40 mL/min
Temperature
50°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
Forced degradation protocol: Reconstitute 1 mg of cefaclor neat standard with 1.0 mL of 1 M HCl. Heat the solution at 60 ºC for 6 hours then neutralize with 1.0 mL of 1 M NaOH. Refrigerate until ready to inject.
Order Items Used in This Application
- 1. 4-Methyl-m-phenylenediamine
- 2. o-Anisidine
- 3. o-Toluidine
- 4. Benzidine
- 5. 4-Chloroaniline
- 6. 3,3'-Dimethoxybenzidine
- 7. 3,3'-Dimethylbenzidine
- 8. 2-Naphthylamine
- 9. 2,4,5-Trimethylaniline
- 10. 4,4'-methylenedio-toluidine
- 11. 4-Aminobiphenyl
- 12. 3,3'-Dichlorobenzidine
- 13. 2,2'-Dichloro-4,4'-methylenedianiline
- 14. o-Aminoazotoluene
Similar Applications
No Similar applications found.