Vitamin A and E on Kinetex EVO C18, APCI Pos/Neg Polarity Switching LC/MS/MS

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4633-AN

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: DI H2O

B: 50:50 IPA:ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

35

65

2

3

5

95

3

4

5

95

4

4.1

35

65

5

5.5

35

65

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (1°C)

Detection Info