FAMEs In Palm Oil by GC/FID on Zebron ZB-FAME

GC Conditions

Column

Zebron ZB-FAME, GC Cap. Column 60m x 0.25mm x 0.2 µm, Ea

Part No

7KG-G033-10

Phase Name

ZB-FAME

Injection

Split 100:1 @ 240°C

Oven Program

180 °C isothermal

Carrier Gas

Constant Flow Helium, 1.2 mL/min

Detection

Flame Ionization (FID) @ (240°C)

Detection Info

Sample Notes

Recommended Accessories: Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID Liner Part No.: AG2-0A11-05 (for Agilent systems) Inlet Seal: AG0-8620 (Gold Plated Easy Seal) Septum: AG0-4696 (PhenoRed-400) Sample Preparation: 1. Weigh 0.2 g of sample into a glass test tube 2. Add 3.8 mL of hexane and vortex 3. Condition SPE tube (Strata® Si-1, 2 g/12 mL, Part No.: 8B-S012-KDG) under vacuum with 6 mL of hexane; do not let bed go dry 4. Load sample with light vacuum 5. Elute with 5 mL of hexane:ethyl acetate (87:13) 6. Evaporate under nitrogen at 35 °C 7. Reconstitute with 4 mL of hexane 8. Add 200 µL of 2 M potassium hydroxide/methanol solution and vortex 9. Leave at room temperature for 5 minutes 10. Add 2 mL of deionized water and vortex 11. Leave to separate until upper layer becomes clear 12. Extract upper layer for GC analysis

Sample Preparation Details

Pretreatment Note

To reconstituted sample, add 200 µL of 2 M potassium hydroxide in methanol, cap tube and vortex. Wat 5 minutes. Add 2 mL Milli-Q® water, vortex. Allow solution to settle then transfer top layer to Q-sert vial for GC analysis.

Condition 1

6 mL Hexane

Load

Load 3.80 mL Sample diluted in Pretreated Sample

Elution 1

5 mL Hexane/Ethyl Acetate (87:13)

Evaporation

Evaporate to dryness

Reconstitute

400 µL Hexane