FAMEs In Canola Oil by GC/MS on Zebron ZB-FAME

GC Conditions

Column

Zebron ZB-FAME, GC Cap. Column 30m x 0.25mm x 0.2 µm, Ea

Part No

7HG-G033-10

Phase Name

No Phase

Injection

Split 50:1 @ 240°C

Oven Program

100 °C for 2 min to 214 °C @ 3 °C/min

Carrier Gas

Constant Flow Helium, 1.0 mL/min

Detection

Mass Spectrometer (MS) @ (250°C)

Detection Info

Sample Notes

Recommended Accessories: Recommended Liner: Zebron PLUS Single Taper Z-Liner, 4 mm ID Liner Part No.: AG2-0A13-05 (for Agilent systems) Inlet Seal: AG0-8620 (Gold Plated Easy Seal) Septum: AG0-4696 (PhenoRed-400) Sample Preparation: 1. Weigh out 25 mg of oil into a 5 mL screw cap glass vial 2. Add 2 mL of BF3-methanol to sample vial 3. Heat vial at 60 °C for 15 minutes with periodic vortexing 4. Remove from heat and allow to attain room temperature 5. Add 1 mL water followed by 1 mL hexane 6. Vortex for 1 minute 7. Remove upper organic layer 8. Dry hexane layer over anhydrous sodium sulfate 9. Transfer to autosampler vial and inject on GC