Peptide mapping by LCMS using Luna Omega 5um PS C18 50x2.1mm

LC Conditions

Column

Luna Omega 5 µm PS C18 100Å, LC Column 50 x 2.1 mm, EA

Brand

Luna Omega

Part No

00B-4753-AN

Phase Name

PS C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% formic acid

B: Acetonitrile with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

50

50

50

Flow Rate

0.50 mL/min

Temperature

25°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info