TCAs on Kinetex 2.6u Polar C18, 50x2.1mm (100 ng/mL)

LC Conditions

Column

Kinetex 2.6 µm Polar C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4759-AN

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 10 mM ammonium formate in water

B: 0.1% formic acid in methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

55

45

2

2

35

65

3

2.5

5

95

4

3

5

95

5

3.1

55

45

6

4.5

55

45

Flow Rate

0.50 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (600°C)

Detection Info