Charge Variants of Trastuzumab using pH Gradient on bioZen 6 µm WCX

LC Conditions

Column

bioZen 6µm WCX, LC Column 250 x 4.6 mm, Ea

Brand

Biozen LC

Part No

00G-4777-E0

Phase Name

WCX

Elution Type

Gradient

Mobile Phase

A: CX-1 , pH 5.6

B: CX-2, pH 10.2

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

1

100

0

3

21

0

100

4

23

0

100

7

24

100

0

Flow Rate

1.00 mL/min

Temperature

25°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/UV (DAD, PDA) @ 280 nm (nanometers)

Detection Info