5a) Sulfonic Acid Esters on Luna Omega Polar C18 - formic acid

LC Conditions

Column

Luna Omega 1.6µm Polar C18 100Å, LC Column 50 x 2.1 mm, EA

Brand

Luna Omega

Part No

00B-4748-AN

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid in water

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

5

10

90

3

5.1

10

90

4

6

98

2

5

7

98

2

Flow Rate

0.60 mL/min

Temperature

25°C

System

Waters ACQUITY UPLC I-Class Waters ACQUITY UPLC I-Class

Detection

LC/UV (DAD, PDA) @ 220 nm (nanometers)

Detection Info