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Applications
Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)
25777
Separation Mode: Reversed Phase
Reversed Phase
PS C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical

Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)

Analytes
15,6 Dihydrouracil (UH2)
2Uracil
3Uracil 1,3-15N2 (+Ve IS)
Details
LC Conditions (App ID: 25777)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
PS C18
System:
Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity
Sample Note:
Add 100 µL Serum (doubly stripped) to tube. Add 900 µL of chilled (~00C) ACN to the serum while vortexing. Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant Analyte diluted with initial mobile phase
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