Gradient Optimization for Intact Mass LC Methods- Gradient Program Length

LC Conditions

Column

bioZen 2.6 µm WidePore C4, LC Column 100 x 2.1 mm, Ea

Brand

Biozen LC

Part No

00D-4786-AN

Phase Name

WIDEPORE C4

Elution Type

Gradient

Mobile Phase

A: 0.1 % Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.9

95

5

2

7.5

10

90

3

7.8

10

90

4

8

95

5

5

8.2

10

90

6

8.3

10

90

7

8.4

95

5

8

12

95

5

Flow Rate

0.50 mL/min

Temperature

80°C

System

Waters ACQUITY UPLC I-Class Waters ACQUITY UPLC I-Class

Detection

LC/UV (DAD, PDA)

Detection Info

Sample Notes

Remicade