Levothyroxine and Impurities F and G according to Ph.Eur. Monograph 0401 on Luna Omega 3 µm C18 150 x 4.6 mm @1.32 mL/min

LC Conditions

Official Method

Ph.Eur. M.401

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Dissolve 1.97 g of phosphoric acid in water and dilute to 2 L with water

B: Dissolve 1.97 g of phosphoric acid in acetonitrile and dilute to 2 L with acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

2

10

70

30

3

40

20

80

4

50

20

80

Flow Rate

1.32 mL/min

Temperature

25°C

System

Shimadzu Shimadzu Nexera UHPLC

Detection

LC/UV (DAD, PDA) @ 225 nm (nanometers)

Detection Info