Diclofenac Sodium Ph.Eur. Reference Solution B System Suitability on Kinetex 5 µm C18 250x4.6mm LC/UV

LC Conditions

Official Method

Ph.Eur. M.1002

Column

Kinetex 5 µm C18 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Kinetex

Part No

00G-4601-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: Mix 34 volumes of a solution containing 0.5 g/L of phosphoric acid R and 0.8 g/L of sodium dihydrogen phosphate R, previously adjusted to pH 2.5 with phosphoric acid R, and 66 volumes of methanol R.

Gradient Profile

Step No.

Time(min)

%A

%B

1

40

100

Flow Rate

1.00 mL/min

Temperature

25°C

System

Agilent Technologies Agilent Technologies 1100 Series

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers)

Detection Info