Lisinopril Ph. Eur. Reference Solution C on Kinetex 5 µm XB-C18 250 x 4.6 mm on Agilent 1100 LC/UV

LC Conditions

Official Method

Ph.Eur. M.1120

Column

Kinetex 5 µm XB-C18 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Kinetex

Part No

00G-4605-E0

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: Mix 3 volumes of acetonitrile R1 and 97 volumes of 3.12 g/L solution of sodium dihydrogen phosphate R previously adjusted to pH 3.8 with dilute phosphoric acid R

B: Mix 20.5 volumes of acetonitrile R1 and 79.5 volumes of 3.12 g/L solution of phosphoric acid R

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

2

2

100

3

37

0

100

4

62

0

100

5

62.5

100

6

66

100

Flow Rate

1.60 mL/min

Temperature

50°C

System

Agilent Technologies Agilent Technologies 1100 Series

Detection

LC/UV (DAD, PDA) @ 210 nm (nanometers)

Detection Info