USP Alosetron Organic Impurities Sensitivity Solution on Gemini 3 μm C18 75 x 4.6 mm using Agilent 1260 Binary UHPLC

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 75 x 4.6 mm, Ea

Brand

Gemini

Part No

00C-4439-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.02 M monobasic sodium phosphate. Adjust with phosphoric acid to a pH of 3.0

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

5

90

10

3

20

50

50

4

25

50

50

5

26

90

10

6

30

90

10

Flow Rate

1.00 mL/min

Temperature

30°C

System

Agilent Technologies Agilent Technologies 1260 Infinity

Detection

LC/UV (DAD, PDA) @ 220 nm (nanometers)

Detection Info