USP Doxepin Assay - Standard Solution on Luna 5 µm C8(2) 150 x 4.6 mm

LC Conditions

Column

Luna 5 µm C8(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4249-E0

Phase Name

C8(2)

Elution Type

Isocratic

Mobile Phase

A: 27.6 g/L monobasic sodium phosphate, pH 2.5

B: methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

Flow Rate

1.32 mL/min

Temperature

50°C

System

Agilent Technologies 1290 Infinity Agilent Technologies 1290 Infinity

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers)

Detection Info

Sample Notes

Standard solution/sample solution: 0.1 mg/mL of USP Doxepin Hydrochloride RS in Mobile phase.