Hydrocortisone Standard Verex Filter Vial-RC filtered with Guard

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4252-E0

Phase Name

C18(2)

Guard Cartridge:

AJ0-4287

Guard Holder:

KJ0-4282

Elution Type

Gradient

Mobile Phase

A: Water

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

2

20

50

50

Flow Rate

1.00 mL/min

Temperature

35°C

System

Agilent Technologies 1290 Infinity Agilent Technologies 1290 Infinity

Detection

LC/UV (DAD, PDA) @ 220 nm (nanometers)

Detection Info

Sample Notes

Hydrocortisone cream