LC-MS/MS Pesticides ( Metolachlor ) - RC Filter in Cumin Exacts for the comparison of with/without Filter Vial-

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 3.0 mm, Ea

Brand

Luna Omega

Part No

00F-4784-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid and 10mM Ammonium Acetate in water

B: 0.5% Formic Acid in Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

0.1

95

5

3

0.5

40

60

4

3.5

25

75

5

8

15

85

6

10.5

5

95

7

12

5

95

8

12.1

95

5

9

18

95

5

Flow Rate

0.40 mL/min

Temperature

20°C

System

Agilent Technologies 1100 Series SCIEX 5500 QTRAP

Detection

LC/MS/MS

Detection Info

Sample Notes

a) Prepared 1mg/mL Metolachlor solutions with other 17 pesticide standards b) Take sample to prepare a 10x Diluted mixture by placing 100uL of the existing mix into a 900uL of a 1:2 Water:Acetonitrile mix. c) Place 30uL of the 10x diluted mixture into 270 uL of the chili powder supernatant. d) Place 450uL of sample from step c) into the filter vial for final analysis.