LC-MS/MS Pesticides ( simazine ) - No Filter in Cumin Exacts for the comparison of with/without Filter Vial

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 3.0 mm, Ea

Brand

Luna Omega

Part No

00F-4784-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid and 10mM Ammonium Acetate in water

B: 0.5% Formic Acid in Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

0.1

95

5

3

0.5

40

60

4

3.5

25

75

5

8

15

85

6

10.5

5

95

7

12

5

95

8

12.1

95

5

9

18

95

5

Flow Rate

0.40 mL/min

Temperature

20°C

System

Agilent Technologies 1100 Series SCIEX 5500 QTRAP

Detection

LC/MS/MS

Detection Info

Sample Notes

a) Prepared 10mg/mL simazine solutions with other 17 pesticide standards b) Take sample to prepare a 10x Diluted mixture by placing 100uL of the existing mix into a 900uL of a 1:2 Water:Acetonitrile mix. c) Place 30uL of the 10x diluted mixture into 270 uL of the cumin powder supernatant for final analysis.