LC-MS/MS Pesticides ( simazine ) - RC Filter in Cumin Exacts for the comparison of with/without Filter Vial-- clone

Analytes

1simazine
2simazine

Compound Name Cid42
CID 42
Molecular Formula C9H10O6
Molecular Weight 214
No. Hydrogen Bond Acceptors 6
No. Hydrogen Bond Donors 3

Details

LC Conditions (App ID: 26539)

Column:

Luna Omega 3 µm C18 100 Å, LC Column 150 x 3.0 mm, Ea

Brand Name:

Luna Omega

Part No:

00F-4784-Y0

Phase Name:

C18

Elution Type:

Gradient

Mobile Phase:

A: 0.1% Formic Acid and 10mM Ammonium Acetate in water

B: 0.5% Formic Acid in Methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	95	5
2	0.1	95	5
3	0.5	40	60
4	3.5	25	75
5	8	15	85
6	10.5	5	95
7	12	5	95
8	12.1	95	5
9	18	95	5

Flow Rate:

0.4 mL/min

Temperature:

20°C

System:

Agilent Technologies 1100 Series Agilent Technologies 1100 Series

Detection:

LC/MS/MS

Sample Note:

a) Prepared 1mg/mL simazine solutions with other 17 pesticide standards b) Take sample to prepare a 10x Diluted mixture by placing 100uL of the existing mix into a 900uL of a 1:2 Water:Acetonitrile mix. c) Place 30uL of the 10x diluted mixture into 270 uL of the chili powder supernatant. d) Place 450uL of sample from step c) into the filter vial for final analysis.

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