USP Ezetimibe Organic Impurities Standard Solution 0.25mg/mL on Kinetex 5u F5 150x4.6mm on Agilent 1260 Quaternary UHPLC system

LC Conditions

Column

Kinetex 5 µm F5 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4724-E0

Phase Name

F5

Elution Type

Gradient

Mobile Phase

A: Water

B: Acetonitrile

C: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

66

24

2

37

66

24

3

60

40

50

4

70

40

50

5

80

10

80

6

90

10

80

7

90.1

66

24

8

100

66

24

Flow Rate

2.00 mL/min

Temperature

30°C

System

Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity

Detection

LC/UV (DAD, PDA) @ 248 nm (nanometers)

Detection Info