USP Atazanavir Sulfate (Assay, Organic Impurities –Procedures 1 and 2, Limit of Atazanavir Related Compound A)​ Atazanavir system suitability solution-Zoomed scale. USP- Organic Impurity-Procedure-1

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 2.73 g/L of monobasic potassium phosphate in water. Adjusted pH with diluted Orthophosphoric acid to pH 3.50. Solution A(MP-A): Acetonitrile and Buffer (25:75) v/v.

B: 2.73 g/L of monobasic potassium phosphate in water. Adjusted pH with diluted Orthophosphoric acid to pH 3.50. Solution B(MP-B) : Acetonitrile and Buffer (75:25) v/v.

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

5

100

0

3

45

0

100

4

50

100

0

5

60

100

0

Flow Rate

1.00 mL/min

Temperature

25°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

UV-Vis Abs.-Diode Array (PDA) @ 215 nm (nanometers)

Detection Info