USP Atazanavir Sulfate (Assay, Organic Impurities–Procedures 1 and 2, Limit of Atazanavir Related Compound A).​Related Compound-A 1µg/ml, chromatogram for USP-Limit of Related compound-A in Atazanavir

LC Conditions

Column

Luna Omega 3 µm Polar C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4760-E0

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 2.73 g/L of monobasic potassium phosphate in water. Adjusted pH to pH 3.50 with diluted Orthophosphoric acid. Acetonitrile and Buffer (25:75) v/v.

B: 2.73 g/L of monobasic potassium phosphate in water. Adjusted pH to pH 3.50 with diluted Orthophosphoric acid. Acetonitrile and Buffer (75:25) v/v.

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5

95

40

3

8

0

100

4

14

0

100

5

15

95

5

6

25

95

5

Flow Rate

1.00 mL/min

Temperature

25°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

UV-Vis Abs.-Diode Array (PDA) @ 215 nm (nanometers)

Detection Info