USP Amlodipine, Valsartan, and Hydrochlorothiazide Tablets –Assay and Impurities-Amlodipine,Valsartan,Hydrochlorothiazide Standard solution-SST Chromatogram USP-Assay
LC Conditions
Column
Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
Brand
Luna Omega
Part No
00F-4784-E0
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 0.1% Orthophosphoric acid in water. (1 ml of Orthophosphoric acid in dissolved in 1000 ml of water. Filtered through 0.22µ Nylon filter. Mobile phase A: Acetonitrile: water and Phosphoric acid in ratio of 50:950:1 ml v/v/v.
B: 0.1% Orthophosphoric acid in water. (1 ml of Orthophosphoric acid in dissolved in 1000 ml of water. Filtered through 0.22µ Nylon filter. Mobile phase B: Acetonitrile: water and Phosphoric acid in ratio of 950:50:1 ml v/v/v.
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
2
3
50
50
3
6
40
60
4
10
5
95
5
10.1
95
5
6
15
95
5
Flow Rate
1.50 mL/min
Temperature
40°C
System
Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class
Detection
LC/UV (DAD, PDA) @ 210
Detection Info
Order Items Used in This Application
- 1. Hydrochlorothiazide
- 2. Amlodipine
- 3. Valsartan
-OEChem-06211003152D<br><br> 25 26 0 0 0 0 0 0 0999 V2000<br> 3.4030 1.2327 0.0000 Cl 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8950 -0.8020 0.0000 S 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 -0.7673 0.0000 S 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3849 -1.6737 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3850 -1.6622 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9030 -1.6333 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.9030 0.0987 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8010 -0.2881 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8950 1.2674 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5369 -1.2673 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -0.2673 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 0.7327 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8010 0.7535 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -0.7673 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -0.2673 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 1.2327 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 0.7327 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.4119 0.6474 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0101 1.3372 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -1.3873 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3368 -0.6002 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8878 1.8873 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 1.8527 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.9573 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5369 -1.8873 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 17 1 0 0 0 0<br> 2 4 2 0 0 0 0<br> 2 5 2 0 0 0 0<br> 2 8 1 0 0 0 0<br> 2 11 1 0 0 0 0<br> 3 6 2 0 0 0 0<br> 3 7 2 0 0 0 0<br> 3 10 1 0 0 0 0<br> 3 15 1 0 0 0 0<br> 8 13 1 0 0 0 0<br> 8 21 1 0 0 0 0<br> 9 12 1 0 0 0 0<br> 9 13 1 0 0 0 0<br> 9 22 1 0 0 0 0<br> 10 24 1 0 0 0 0<br> 10 25 1 0 0 0 0<br> 11 12 1 0 0 0 0<br> 11 14 2 0 0 0 0<br> 12 16 2 0 0 0 0<br> 13 18 1 0 0 0 0<br> 13 19 1 0 0 0 0<br> 14 15 1 0 0 0 0<br> 14 20 1 0 0 0 0<br> 15 17 2 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 23 1 0 0 0 0<br>M END
Compound Name
Hydrochlorothiazide
CID
3639
Molecular Formula
C7H8ClN3O4S2
Molecular Weight
297
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
3
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