HILIC Separation of Nucleobases using a Diol HILIC column
LC Conditions
Column
Luna Omega 3µm HILIC 100Å, LC Column 150 x 4.6 mm, ea
Brand
Luna Omega
Part No
00F-4808-E0
Phase Name
HILIC
Elution Type
Isocratic
Mobile Phase
A: 100 mM Ammonium Formate, pH 3.2
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
10
90
Flow Rate
1.00 mL/min
Temperature
22°C
System
Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class
Detection
LC/UV (DAD, PDA) @ 260 nm (nanometers)
Detection Info
Order Items Used in This Application
- 1. Thymine
- 2. Uracil
- 3. Adenine
- 4. Cytosine
- 5. Guanine
-OEChem-06211003142D<br><br> 15 15 0 0 0 0 0 0 0999 V2000<br> 5.4641 -0.6900 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.6900 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -0.6900 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 0.8100 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 0.8100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -0.1900 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.3100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 1.3100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -0.1900 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.9300 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -1.3100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3291 1.1200 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7741 0.7731 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 1.6200 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1541 1.8469 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 6 2 0 0 0 0<br> 2 9 2 0 0 0 0<br> 3 6 1 0 0 0 0<br> 3 9 1 0 0 0 0<br> 3 11 1 0 0 0 0<br> 4 7 1 0 0 0 0<br> 4 9 1 0 0 0 0<br> 4 12 1 0 0 0 0<br> 5 6 1 0 0 0 0<br> 5 7 2 0 0 0 0<br> 5 8 1 0 0 0 0<br> 7 10 1 0 0 0 0<br> 8 13 1 0 0 0 0<br> 8 14 1 0 0 0 0<br> 8 15 1 0 0 0 0<br>M END
Compound Name
Thymine
CID
1135
Molecular Formula
C5H6N2O2
Molecular Weight
126
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
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