Amino Acids - OPA Derivatives

LC Conditions

Column

Luna 3 µm C18(2) 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Luna

Part No

00D-4251-E0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: 0.1M Na2HPO4, anhydrous and 0.1 mM Na2-EDTA dihydrate in water, pH 6.38

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

Flow Rate

1.20 mL/min

Temperature

40°C

Detection

Fluorescence (FLUOR) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282