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Optimizing Gel Permeation Chromatography (GPC) using Phenogel GPC/SEC Columns
Gel Permeation Chromatography (GPC) is principally used to separate polymers and organic molecules based on their size. An important aspect in optimizing GPC separations is column selection, especially when multiple columns are used. Mobile phase selection and column temperature are also important factors that must be considered when developing a method, as they can influence the solubility and viscosity of diluent and analyte respectively. Column, mobile phase, and temperature selection will be discussed to optimize GPC methods using Phenogel GPC/SEC columns.
Optimizing Gel Permeation Chromatography (GPC) using Phenogel GPC/SEC Columns
Gel Permeation Chromatography (GPC) is principally used to separate polymers and organic molecules based on their size. An important aspect in optimizing GPC separations is column selection, especially when multiple columns are used. Mobile phase selection and column temperature are also important factors that must be considered when developing a method, as they can influence the solubility and viscosity of diluent and analyte respectively. Column, mobile phase, and temperature selection will be discussed to optimize GPC methods using Phenogel GPC/SEC columns.
Document Type:
Technical Notes
Brands:
Separation Modes:
Size Exclusion
Phases:
Optimizing Gel Permeation Chromatography (GPC) using Phenogel GPC/SEC Columns
Gel Permeation Chromatography (GPC) is principally used to separate polymers and organic molecules based on their size. An important aspect in optimizing GPC separations is column selection, especially when multiple columns are used. Mobile phase selection and column temperature are also important factors that must be considered when developing a method, as they can influence the solubility and viscosity of diluent and analyte respectively. Column, mobile phase, and temperature selection will be discussed to optimize GPC methods using Phenogel GPC/SEC columns.
| Target Industries: | Biopharmaceutical Analysis, Chemical and Energy, Environmental Testing, Food Testing. Food Safety & Food Quality Analysis., Pharmaceutical |
| Techniques: | HPLC, HPLC-SEC |
| Brands: | Phenogel |
| Separation Modes: | Size Exclusion |
| Phases: | Phenogel 100 Å , Phenogel 500 Å , Phenogel 50 Å , Phenogel Linear(2) |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.