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Native MS for Aggregate and Charge Variant Analysis for Biotherapeutics

Size exclusion chromatography (SEC) and ion exchange chromatography (IEX) are classic techniques employed in the characterization of biotherapeutics under non-denaturing conditions. Standard mobile-phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native MS represents a cutting-edge approach to employ MS-compatible mobile phases for non-denaturing SEC and IEX analysis. Learn about aggregate and charge-variant analysis using native-mode MS, including understanding the fundamentals in SEC and IEX for biotherapeutic characterization, the effect of mobile-phase conditions and chromatographic parameters in aggregate analysis by native SEC, and determination of acidic and basic variants of monoclonal antibodies using native weak cation exchange chromatography (WCX).