Simultaneous Detection of Tricarboxylic Acid Cycle Intermediates using LC-MS/MS with a Synergi Fusion-RP HPLC Column (TN-1241)

In this technical note, the goal was to develop an LC-MS/MS assay able to analyze tricarboxylic acids out of a human serum albumin matrix over a dynamic range of concentrations and demonstrate acceptable accuracy and precision in reference to GLP guidance. In addition, the reference method should be a fast and reproducible assay, easily implemented in most laboratories with standard analytical equipment.

Document Type:

Technical Notes

Target Industries:

Biopharmaceutical Analysis, Clinical Research, Pharmaceutical

Techniques:

HPLC, HPLC Reversed Phase

Brands:

Synergi

Separation Modes:

Reversed Phase

Phases:

Synergi Fusion-RP